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AB29545

HeLa whole cell lysate

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(3 Publications)

HeLa whole cell lysate (ab29545) suitable for Western blot and SDS-PAGE assays.

- Total protein is prepared from whole tissue homogenates
- Storage buffer contains protease inhibitor cocktail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Form

Liquid

form

Disease

Adenocarcinoma

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

Product details

Protein is isolated from HeLa cell pellet using a proprietary technique. <br><br>The pellet was frozen in liquid nitrogen immediately and then stored at -70°C.

Quality control
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody.
The expression level is consistent with each lot.

View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Allow to warm to room temp and agitate gently before aliquoting|Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

G3 (Bethesda, Md.) 15: PubMed40519079

2025

Characterizing fatty acid oxidation genes in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Juliana Geronazzo,Abigail Heimerl,Linnea Lindell,Skye McCrimmon,Clara Stormer,Brooke Horvai,Ian P Johnson,Tia M Peterson,Jocelyn Zuckerman,Anna I Scott,Meredith M Course

Scientific reports 11:8178 PubMed33854169

2021

H3K4me2 ChIP-Seq reveals the epigenetic landscape during mushroom formation and novel developmental regulators of Schizophyllum commune.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Jan Vonk,Robin A Ohm

Investigative ophthalmology & visual science 54:1887-97 PubMed23422828

2013

L450W and Q455K Col8a2 knock-in mouse models of Fuchs endothelial corneal dystrophy show distinct phenotypes and evidence for altered autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Meng,Mario Matthaei,Narendrakumar Ramanan,Rhonda Grebe,Shukti Chakravarti,Caroline L Speck,Martha Kimos,Neeraj Vij,Charles G Eberhart,Albert S Jun
View all publications

Product promise

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