HeLa whole cell lysate (IL1 alpha stimulated)
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Functional Studies - HeLa whole cell lysate (IL1 alpha stimulated) (AB14656)
ab14656 tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.
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Product details
The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without IL1 alpha (5 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
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