Hep G2 nuclear extract lysate

13 Images

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-PROX1 antibody [5G10] (<a href='/en-us/products/primary-antibodies/prox1-antibody-5g10-ab33219'>ab33219</a>) at 10 µg/mL

Lane 1:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

true

Exposure time: 20min

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

ab42389 detects a band at 80 kDa which corresponds to the NCBP 80 kDa subunit.

All lanes:

Western blot - Anti-CBP80 antibody (<a href='/en-us/products/primary-antibodies/cbp80-antibody-ab42389'>ab42389</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Lane 7:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 80 kDa

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-FOXA2 antibody (<a href='/en-us/products/primary-antibodies/foxa2-antibody-ab23630'>ab23630</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 48 kDa

Observed band size: 140 kDa,52 kDa

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-CDCA2 antibody (<a href='/en-us/products/primary-antibodies/cdca2-antibody-ab45129'>ab45129</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 113 kDa

Observed band size: 125 kDa,40 kDa

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-TDP43 antibody (<a href='/en-us/products/primary-antibodies/tdp43-antibody-ab41881'>ab41881</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Lane 7:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

K-562 (Human) Nuclear Lysate - tumor cell line (<a href='/en-us/products/unavailable/k-562-human-nuclear-lysate-tumor-cell-line-ab29309'>ab29309</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 44 kDa

Observed band size: 47 kDa

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-JDP2 antibody (<a href='/en-us/products/primary-antibodies/jdp2-antibody-ab40916'>ab40916</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Lane 6:

Du145 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 18 kDa

Observed band size: 19 kDa

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-BRG1 antibody (<a href='/en-us/products/primary-antibodies/brg1-antibody-ab4081'>ab4081</a>) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 185 kDa

Observed band size: 235 kDa,40 kDa

false

• WB

Lab

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-cbx7 antibody (<a href='/en-us/products/primary-antibodies/cbx7-antibody-ab21873'>ab21873</a>) at 1 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 3:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 28 kDa

Observed band size: 100 kDa,34 kDa,70 kDa

true

Exposure time: 30s

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-cbx7 antibody (<a href='/en-us/products/primary-antibodies/cbx7-antibody-ab21873'>ab21873</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

Predicted band size: 28 kDa

Observed band size: 100 kDa,34 kDa,70 kDa

false

• WB

Unknown

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (<a href='/en-us/products/primary-antibodies/tata-binding-protein-tbp-antibody-nuclear-loading-control-and-chip-grade-ab63766'>ab63766</a>) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Testis (Rat) Tissue Lysate at 10 µg

Lane 4:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa,45 kDa,55 kDa

true

Exposure time: 3min

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Anti-MDC1 (phospho T4) antibody (<a href='/en-us/products/unavailable/mdc1-phospho-t4-antibody-ab35967'>ab35967</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Lane 3:

Hep G2 (Human) Nuclear Lysate - IFN gamma treated (<a href='/en-us/products/unavailable/hep-g2-human-nuclear-lysate-ifn-gamma-treated-ab14662'>ab14662</a>) at 10 µg

Lane 4:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg with Human MDC1 (phospho T4) peptide (<a href='/en-us/products/proteins-peptides/human-mdc1-phospho-t4-peptide-ab36513'>ab36513</a>)

Lane 5:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg with Human MDC1 (phospho T4) peptide (<a href='/en-us/products/proteins-peptides/human-mdc1-phospho-t4-peptide-ab36513'>ab36513</a>)

Lane 6:

Hep G2 (Human) Nuclear Lysate - IFN gamma treated (<a href='/en-us/products/unavailable/hep-g2-human-nuclear-lysate-ifn-gamma-treated-ab14662'>ab14662</a>) at 10 µg with Human MDC1 (phospho T4) peptide (<a href='/en-us/products/proteins-peptides/human-mdc1-phospho-t4-peptide-ab36513'>ab36513</a>)

Lane 7:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg with Human MDC1 (unmodified ) peptide (<a href='/en-us/products/unavailable/human-mdc1-unmodified-peptide-ab36514'>ab36514</a>)

Lane 8:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg with Human MDC1 (unmodified ) peptide (<a href='/en-us/products/unavailable/human-mdc1-unmodified-peptide-ab36514'>ab36514</a>)

Lane 9:

Hep G2 (Human) Nuclear Lysate - IFN gamma treated (<a href='/en-us/products/unavailable/hep-g2-human-nuclear-lysate-ifn-gamma-treated-ab14662'>ab14662</a>) at 10 µg with Human MDC1 (unmodified ) peptide (<a href='/en-us/products/unavailable/human-mdc1-unmodified-peptide-ab36514'>ab36514</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

false

• WB

Project

Western blot - Hep G2 nuclear extract lysate (AB14660)

All lanes:

Western blot - Anti-CtIP antibody (<a href='/en-us/products/primary-antibodies/ctip-antibody-ab38016'>ab38016</a>) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 101 kDa

Observed band size: 110 kDa,25 kDa,250 kDa

false

• WB

Unknown

Western blot - Hep G2 nuclear extract lysate (AB14660)

Thrombin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Thrombin antibody [5G9] (<a href='/en-us/products/primary-antibodies/thrombin-antibody-5g9-ab17199'>ab17199</a>) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human Plasma Total Protein Lysate at 10 µg

Lane 3:

Western blot - Hep G2 nuclear extract lysate (ab14660) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 70 kDa

Observed band size: 40 kDa,75 kDa

true

Exposure time: 12min