HL-60 whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- Cells 12:2023Influence of the Anesthetic Technique on Circulating Extracellular Vesicles in Bladder Cancer Patients Undergoing Radical Cystectomy: A Prospective, Randomized Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuisa Gluth,Crista Ochsenfarth,Phuong Nam Viet Pham,Jan M Wischermann,Thomas Komanek,Florian Roghmann,Ulrich H FreyPubMed 37887347
- PloS one 18:e02862782023Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSevda T Boyanova,Ethlyn Lloyd-Morris,Christopher Corpe,Khondaker Miraz Rahman,Doaa B Farag,Lee K Page,Hao Wang,Alice L Fleckney,Ariana Gatt,Claire Troakes,Gema Vizcay-Barrena,Roland Fleck,Suzanne J Reeves,Sarah A ThomasPubMed 37874822
- American journal of respiratory cell and molecular 40:200-102008Regulation of bacteria-induced intercellular adhesion molecule-1 by CCAAT/enhancer binding proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLori J Manzel et. al.PubMed 18703796
Key facts
- Cell type
- HL-60
- Species or organism
- Human
- Form
- Liquid
Recommended products
HL-60 whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
- HL-60
- Species or organism
- Human
- Form
- Liquid
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cell line: HL60 (Human promyelocytic leukemia).
Growth media: RPMI and 10% FBS (Fetal bovine serum).
HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
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1 product image
Western blot - HL-60 whole cell lysate (ab7914)
Anti-Cdc20 antibody ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands.
All lanes: Western blot - Anti-Cdc20 antibody (Anti-Cdc20 antibody ab26483) at 1 µg/mL
Lane 1: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908) at 20 µg
Lane 2: Western blot - THP-1 whole cell lysate (THP-1 whole cell lysate ab7913) at 20 µg
Lane 3: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg
Lane 4: Western blot - HL-60 whole cell lysate (ab7914) at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
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