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AB261680

Human ABCC1 (MRP1) knockout A549 cell lysate

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ABCC1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 99%.

View Alternative Names

ABC 29, ABCC, ABCC 1, ATP binding cassette transporter variant ABCC1delta ex13, ATP binding cassette transporter variant ABCC1delta ex13&14, ATP binding cassette transporter variant ABCC1delta ex25, ATP binding cassette transporter variant ABCC1delta ex25&26, ATP binding cassette, sub-family C (CFTR/MRP), member 1, ATP-binding cassette sub-family C member 1, DKFZp686N04233, DKFZp781G125, GS X, LTC4 transporter, Leukotriene C(4) transporter, MRP1_HUMAN, Multidrug resistance protein, Multidrug resistance-associated protein 1, Multiple drug resistance associated protein, Multiple drug resistance protein 1

4 Images
Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)
  • WB

Lab

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2 : ABCC1 knockout A549 (Human lung carcinoma cell line) whole cell lysate

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lanes 1 - 4 : Merged signal (red and green). Green - ab99531 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab99531 was shown to specifically react with MRP1 in wild-type A549 cells as signal was lost in ABCC1 knockout cell line ab261871 (knockout cell lysate ab261680). Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab99531 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-MRP1 antibody (<a href='/en-us/products/unavailable/mrp1-antibody-ab99531'>ab99531</a>) at 1 µg/mL

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

ABCC1 knockout A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

Western blot - Human ABCC1 (MRP1) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-abcc1-mrp1-knockout-a549-cell-line-ab261871'>ab261871</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

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Next Generation Sequencing - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)
  • NGS

Lab

Next Generation Sequencing - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)

X = 11 bp deletion

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)
  • WB

Lab

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lane 2 : MRP1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lane 3 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lane 4 : MRP1 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lanes 1-4 : Merged signal (red and green). Green - ab233383 observed at 250 kDa. Red - loading control ab7291 observed at 50 kDa.
ab233383 Anti-MRP1 antibody [EPR21062] was shown to specifically react with MRP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261871 (knockout cell lysate ab261680) was used. Wild-type and MRP1 knockout samples were subjected to SDS-PAGE. ab233383 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP1 antibody [EPR21062] (<a href='/en-us/products/primary-antibodies/mrp1-antibody-epr21062-ab233383'>ab233383</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC1 (MRP1) knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-abcc1-mrp1-knockout-hela-cell-lysate-ab257242'>ab257242</a>) at 20 µg

Lane 3:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (ab261680) at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 171 kDa

Observed band size: 250 kDa,50 kDa

false

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)
  • WB

Supplier Data

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (AB261680)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2 : ABCC1 knockout A549 (Human lung carcinoma cell line) whole cell lysate

Lanes 1 - 2 : Merged signal (red and green). Green - ab180960 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab180960 was shown to specifically react with ABCC1 in wild-type A549 cells as signal was lost in ABCC1 knockout cell line ab261871 (knockout cell lysate ab261680). Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180960 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/mrp1-antibody-epr46582-c-terminal-ab180960'>ab180960</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (ab261680) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 171 kDa

Observed band size: 171 kDa,130 kDa

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Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 99%

Disease

Carcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ABCC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.
Biological function summary

This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.

Pathways

MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.

MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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