Skip to main content

ABCC2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 97%.

Be the first to review this product! Submit a review

Images

Western blot - Human ABCC2 knockout A549 cell lysate (AB261663), expandable thumbnail
  • Western blot - Human ABCC2 knockout A549 cell lysate (AB261663), expandable thumbnail
  • Western blot - Human ABCC2 knockout A549 cell lysate (AB261663), expandable thumbnail
  • Western blot - Human ABCC2 knockout A549 cell lysate (AB261663), expandable thumbnail
  • Next Generation Sequencing - Human ABCC2 knockout A549 cell lysate (AB261663), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Knockout validation
Next Generation Sequencing, Western blot
Mutation description
Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 97%

Alternative names

ABC30, ATP binding cassette sub family C (CFTR/MRP) member 2, ATP-binding cassette sub-family C member 2, CMOAT, CMOAT1, Canalicular multidrug resistance protein, Canalicular multispecific organic anion transporter 1, DJS, KIAA1010, MRP2_HUMAN, Multidrug resistance-associated protein 2, abcC2, cMRP

What's included?

1 Kit
Components
Human ABCC2 knockout A549 cell lysate
1 x 100 µg
Human wild-type A549 cell lysate
1 x 100 µg

Recommended products

ABCC2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 97%.

Key facts

Cell type
A549
Mutation description
Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 97%
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
ABCC2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRP2 also known as ABCC2 or MRP-2 transporter is an ATP-binding cassette transporter. This protein with a molecular mass of about 190 kDa is widely expressed in tissues such as the liver kidney and intestine. MRP2 plays a role in drug and metabolite transport moving various organic anions across cellular membranes. It locates to the canalicular membrane of hepatocytes where it functions in secreting conjugated bilirubin and drugs into bile.

Biological function summary

MRP2 contributes to cellular detoxification processes. It does not form part of a larger protein complex but works as an independent entity. MRP2 protein actively transports conjugated substances out of cells protecting tissues from potential damage from toxins drugs and other organic anions. This transporter mediates the excretion of glucuronide sulfate and glutathione conjugates playing an important role in phase III of drug metabolism.

Pathways

MRP2 transporter is integral to xenobiotic metabolism. It interconnects with the conjugation pathways that prepare compounds for excretion. The pathway functions with cytochrome P450 enzymes and other related transport proteins like MDR1 facilitating the secretion of detoxified metabolites. MRP2 activity influences pharmacokinetics and pharmacodynamics by modulating drug disposition and excretion.

Associated diseases and disorders

MRP2 is associated with Dubin-Johnson syndrome and cholestasis. Dubin-Johnson syndrome arises from mutations in the ABCC2 gene leading to defective bilirubin excretion and jaundice. Cholestasis involves impaired bile excretion where MRP2 and possibly related proteins like BSEP play roles in bile salt export. Understanding MRP2 functioning aids in assessing risk and treatment strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com