Human ABCC3 (MRP3) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
ABCC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 2 bp deletion in exon 4.
View Alternative Names
ABC 31, ABCC 3, ATP binding cassette sub family C (CFTR/MRP) member 3, ATP-binding cassette sub-family C member 3, CMOAT 2, Canalicular multispecific organic anion transporter 2, Canicular multispecific organic anion transporter, EST90757, MLP 2, MOAT-D, MRP3_HUMAN, Multi-specific organic anion transporter D, Multidrug resistance associated protein, Multidrug resistance-associated protein 3, Multispecific organic anion tranporter D
- Sanger seq
Unknown
Sanger Sequencing - Human ABCC3 (MRP3) knockout HeLa cell lysate (AB258292)
Allele-1 : 2 bp deletion in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human ABCC3 (MRP3) knockout HeLa cell lysate (AB258292)
Allele-2 : 1 bp insertion in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRP3 functions in the transport of organic anions across cellular membranes. It does not operate in isolation often interacting within a network of transporters to move glucuronidated sulfated and other conjugated organic anions from the cells. This protein plays a significant role in the body's detoxification processes where it assists in duly removing harmful metabolites and drugs. The MRP3's ability to transport bile acids and conjugates suggests it influences lipid metabolism as well.
Pathways
MRP3 is part of the glutathione and bile acid recycling pathways. Through these pathways MRP3 serves to recycle and transport surplus bile acids from the liver to the blood. This function connects MRP3 with other proteins like BSEP and MRP2 which also deal in bile acid transport. By regulating these pathways MRP3 maintains and controls the homeostasis of essential molecules within the cellular environment.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Functional Studies - Anti-MRP3 antibody [M3II-9] (AB3375)](https://content.abcam.com/products/images/mrp3-antibody-m3ii-9-ab3375--functional-studies-img79510.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com