Human ABCF2 knockout HEK-293T cell lysate
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ABCF2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.
View Alternative Names
0710005O05Rik, ABC28, ATP binding cassette sub family F (GCN20) member 2, DKFZp586K1823, Drr3, E430001O06, EST133090, HUSSY 18, MABC1, MGC128735, MGC53054, fb04f06, fc74f03, fd58d07, wu:fb04f06, wu:fc74f03, wu:fd58d07
- WB
Unknown
Western blot - Human ABCF2 knockout HEK-293T cell lysate (AB258773)
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2 : ABCF2 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
ab50807 was shown to specifically react with ABCF2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266516 (knockout cell lysate ab258773) was used. Wild-type and ABCF2 knockout samples were subjected to SDS-PAGE. ab50807 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ABCF2 antibody [2001C1] (<a href='/en-us/products/primary-antibodies/abcf2-antibody-2001c1-ab50807'>ab50807</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
ABCF2 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
Western blot - Human ABCF2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-abcf2-knockout-hek-293t-cell-line-ab266516'>ab266516</a>)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ABCF2 knockout HEK-293T cell lysate (AB258773)
Allele-1 : 5 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human ABCF2 knockout HEK-293T cell lysate (AB258773)
Allele-2 : 1 bp insertion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ABCF2 impacts translation regulation and stress response through different cellular processes. This protein functions independently and may also participate in complexes with other proteins. Through mechanisms not fully understood ABCF2 associates with cellular stress granules that form in response to environmental stress. These interactions suggest roles in protein synthesis regulation during stress which indirectly influences cell survival and adaptation.
Pathways
ABCF2 integrates into cellular processes related to stress and translation. It intersects with pathways like eukaryotic initiation factor 2 (EIF2) signaling which helps control translation initiation during stress. ABCF2 shares pathway interactions with proteins such as EIF2B and other ribosomal elements indicating a connection to translation regulation. The involvement in ribosomal function places ABCF2 within important cellular response networks especially under conditions that affect protein synthesis fidelity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-ABCF2 antibody [2001C1] (AB50807)](https://content.abcam.com/products/images/abcf2-antibody-2001c1-ab50807--western-blot-img5645.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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