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AB259778

Human ABCG2 knockout A549 cell lysate

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ABCG2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift = 90%.

View Alternative Names

ABC transporter, ABC15, ABCG2_HUMAN, ABCP, ATP binding cassette sub family G (WHITE) member 2, ATP binding cassette transporter G2, ATP-binding cassette sub-family G member 2, BCRP, BCRP 1, BMDP, Breast cancer resistance protein, CD338, CDw338, CDw338 antigen, EST157481, GOUT1, MGC102821, MRX, MXR, MXR1, Mitoxantrone resistance-associated protein, Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2, Placenta specific MDR protein, Placenta-specific ATP-binding cassette transporter, UAQTL1

2 Images
Western blot - Human ABCG2 knockout A549 cell lysate (AB259778)
  • WB

Lab

Western blot - Human ABCG2 knockout A549 cell lysate (AB259778)

Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : ABCG2 knockout A549 cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : THP-1 cell lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - ab207732 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab207732 was shown to react with BCRP/ABCG2 in wild-type A549 cells in Western blot with loss of signal observed in ABCG2 knockout cell line ab259773 (ABCG2 knockout cell lysate ab259778). Wild-type A549 and ABCG2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab207732 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (<a href='/en-us/products/primary-antibodies/bcrp-abcg2-antibody-epr20080-ab207732'>ab207732</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ABCG2 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ABCG2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-abcg2-knockout-a549-cell-line-ab259773'>ab259773</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 72 kDa

Observed band size: 70 kDa

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Next Generation Sequencing - Human ABCG2 knockout A549 cell lysate (AB259778)
  • NGS

Lab

Next Generation Sequencing - Human ABCG2 knockout A549 cell lysate (AB259778)

X = 1 bp deletion

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift = 90%

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ABCG2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Complementary Products

Primary Antibodies

AB207732

Anti-BCRP/ABCG2 antibody [EPR20080]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)

  • 4
  • 6
Primary Antibodies

AB170904

Anti-P Glycoprotein antibody [EPR10364-57]

RabMAb|Recombinant|KO Validated

Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904)

  • 4
  • 12
Primary Antibodies

AB260038

Anti-MRP1 antibody [EPR22841-78]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (AB260038)

  • 0
  • 0
Cell Lines & Lysates

AB259773

Human ABCG2 knockout A549 cell line

Advanced Validation

Western blot - Human ABCG2 knockout A549 cell line (AB259773)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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