Human ABHD6 knockout HeLa cell lysate
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ABHD6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon5 and 2 bp deletion in exon5 and 5 bp deletion in exon5.
View Alternative Names
0610041D24Rik, 2 arachidonoylglycerol hydrolase, AA673485, AV065425, Abhydrolase domain containing 6, Abhydrolase domain containing protein 6, Llipase protein, MGC94917
- Sanger seq
Unknown
Sanger Sequencing - Human ABHD6 knockout HeLa cell lysate (AB258293)
Allele-1 : 5 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human ABHD6 knockout HeLa cell lysate (AB258293)
Allele-2 : 2 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human ABHD6 knockout HeLa cell lysate (AB258293)
Allele-3 : 1 bp deletion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ABHD6 affects endocannabinoid signaling by breaking down 2-AG into arachidonic acid and glycerol. This process positions ABHD6 as an essential regulator of cellular lipid balance and signaling. It is not typically part of a larger complex; ABHD6 operates mainly as a single enzyme. However its interaction with lipids impacts diverse cellular mechanisms including those related to metabolism and neural activity.
Pathways
The enzyme significantly affects the endocannabinoid and lipid metabolism pathways. ABHD6 is involved in modulating the levels of 2-AG influencing neurotransmission and inflammation. It sits within the same metabolic framework as other enzymes like monoacylglycerol lipase (MAGL) and diacylglycerol lipase both of which are important in the lipid signaling pathway. These interactions emphasize the role of ABHD6 in maintaining cellular homeostasis and influencing various physiological processes through lipid metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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