Human ACO1 knockout U-2 OS cell lysate
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ACO1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%.
View Alternative Names
ACO 1, ACOC_HUMAN, ACONS, Aconitase, Aconitase 1 soluble, Aconitase1, Aconitate hydratase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRE-BP 1, IREB 1, IREBP, IRP 1, Iron regulatory protein 1, Iron-responsive element-binding protein 1, OTTHUMP00000045233
- WB
Lab
Western blot - Human ACO1 knockout U-2 OS cell lysate (AB261693)
Lane 1 : Wild-type U-2 OS whole cell lysate 20 ug
Lane 2 : ACO1 knockout U-2 OS whole cell lysate 20 ug
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183721 was shown to specifically react with ACO1 in wild-type U-2 OS cells as signal was lost in ACO1 knockout cell line ab261884 (knockout cell lysate ab261693). Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (<a href='/en-us/products/primary-antibodies/aconitase-1-aco1-antibody-epr72262-ab183721'>ab183721</a>) at 1 µg/mL
Lane 1:
Wild-type U20S whole cell lysate at 20 µg
Lane 2:
ACO1 knockout U-2 OS whole cell lysate at 20 µg
Lane 2:
Western blot - Human ACO1 knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-aco1-knockout-u-2-os-cell-line-ab261884'>ab261884</a>)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4:
HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Predicted band size: 98 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human ACO1 knockout U-2 OS cell lysate (AB261693)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ACO1 connects energy production and iron regulation. It binds to iron-responsive elements when the iron is scarce and acts in its aconitase form when iron levels are sufficient. ACO1 is not part of a larger protein complex but plays an important regulatory role in balancing cellular energy output and iron homeostasis. It affects both glycolysis and the electron transport chain indirectly by altering the availability of citric acid cycle intermediates which are important for ATP production.
Pathways
ACO1 integrates into both the citric acid cycle and the iron regulatory pathway. The citric acid cycle also called the Krebs cycle is fundamental for ATP production and ACO1 serves as a pivotal point within this cycle. Moreover through its iron-regulatory function it connects to iron metabolism pathways that are vital for various cellular processes. ACO1 activity also influences other proteins like ferritin by regulating iron storage and transport mechanisms through its dual functionality.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB183721
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)](https://content.abcam.com/products/images/aconitase-1-aco1-antibody-epr72262-ab183721--western-blot-img140729.jpg)
- 0
- 0
![Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (AB110321)](https://content.abcam.com/products/images/aconitase-2-antibody-6f12bd9-ab110321--immunocytochemistry-immunofluorescence-img53278.gif)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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