Human ACOT9 (Acyl-CoA Thioesterase 9) knockout HeLa cell lysate
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ACOT9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
ACOT9_HUMAN, Acyl-CoA thioester hydrolase 9, Acyl-CoA thioesterase 9, Acyl-coenzyme A thioesterase 9, Acyl-coenzyme A thioesterase 9, mitochondrial, CGI 16, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human ACOT9 (Acyl-CoA Thioesterase 9) knockout HeLa cell lysate (AB258776)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ACOT9 participates in the regulation of lipid metabolism and energy homeostasis. It does not form part of a known complex but performs a stand-alone role in converting activated fatty acids into their free form and CoA. This process contributes to the balance between synthesis and degradation of lipids impacting energy production and storage. ACOT9's action influences cellular metabolic states and can adapt them depending on the energy requirement of the organism.
Pathways
ACOT9 plays an important role in the peroxisomal and mitochondrial fatty acid oxidation pathways. In these pathways it interacts with enzymes such as Acyl-CoA oxidase and Carnitine palmitoyltransferase I facilitating the breakdown of long-chain fatty acids. ACOT9's hydrolytic activity helps reduce the build-up of acyl-CoA intermediates which is essential for maintaining cellular energy balance and supporting metabolic flexibility during fasting or exercise.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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