Human ACOX3 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human ACOX3 knockout HeLa cell lysate (AB258777)
Allele-2 : 5 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human ACOX3 knockout HeLa cell lysate (AB258777)
Allele-1 : 16 bp deletion in exon5
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ACOX3 contributes to the breakdown of pristanic acid a branched-chain fatty acid derived from phytanic acid. It functions as part of a peroxisomal enzyme system that efficiently oxidizes fatty acids that contain branches on their carbon chains which mitochondria can’t oxidize. ACOX3 works alongside other enzymes like ACOX1 and ACOX2 which oxidize different substrates ensuring a specialized fatty acid metabolism in the cell.
Pathways
ACOX3 plays a critical role in the peroxisomal fatty acid beta-oxidation pathway also contributing to the catabolism of very-long-chain fatty acids. This pathway complements the mitochondrial beta-oxidation providing a synergistic approach for complete fatty acid degradation. ACOX3 works closely related to proteins like peroxisomal bifunctional enzyme and thiolase completing the sequential steps necessary for beta-oxidation and energy production from fatty acids.
Quality control
STR analysis
D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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