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AB261668

Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate

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ACP1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%.

View Alternative Names

ACP1, Acid phosphatase 1 soluble, Acid phosphatase of erythrocyte, Adipocyte acid phosphatase, Cytoplasmic phosphotyrosyl protein phosphatase, HAAP, LMW-PTP, LMW-PTPase, Low molecular weight cytosolic acid phosphatase, Low molecular weight phosphotyrosine protein phosphatase, PAP1, PAP2, PPAC_HUMAN, PTPase, Protein tyrosine phosphatase, Purple acid phosphatase, Red cell acid phosphatase 1, phosphatase, acid, of erythrocyte, testicular secretory protein Li 37

5 Images
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)
  • WB

Lab

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab235448 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab235448 was shown to specifically react with ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost in ACP1 knockout cell line ab261859 (knockout cell lysate ab261668). Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab235448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase antibody [EPR21787] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-antibody-epr21787-ab235448'>ab235448</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

false

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)
  • WB

Lab

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cell line ab261859 (knockout cell lysate ab261668). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab166896 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-acp1-antibody-epr9839-ab166896'>ab166896</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)
  • WB

Lab

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab180524 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180524 was shown to specifically react with ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost in ACP1 knockout cell line ab261859 (knockout cell lysate ab261668). Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180524 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-Acid phosphatase/ACP1 antibody [EPR9838(2)] (<a href='/en-us/products/unavailable/acid-phosphataseacp1-antibody-epr98382-ab180524'>ab180524</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

false

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)
  • WB

Lab

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout cell line ab261859 (knockout cell lysate ab261668). Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab235449 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase antibody [EPR21791] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-antibody-epr21791-ab235449'>ab235449</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Next Generation Sequencing - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)
  • NGS

Lab

Next Generation Sequencing - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (AB261668)

X = 1 bp insertion

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ACP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary

The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.

Pathways

The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.

Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB235448

Anti-Acid phosphatase antibody [EPR21787]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21787] (AB235448)

  • 0
  • 0
Assay Kits

AB83367

Acid Phosphatase Assay Kit (Colorimetric)

Functional Studies - Acid Phosphatase Assay Kit (Colorimetric) (AB83367)

  • 0
  • 0
Cell Lines & Lysates

AB261859

Human ACP1 (Acid phosphatase) knockout HEK-293 cell line

Advanced Validation

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

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