Skip to main content

ACVR1B KO cell lysate available now. Free of charge wild type control included.

Be the first to review this product! Submit a review

Key facts

Cell type
THP-1
Species or organism
Human
Tissue
Blood
Knockout validation
Sanger Sequencing

Alternative names

What's included?

1 Kit
Components
Human ACVR1B knockout THP-1 cell lysate
1 x 100 µg
Human ACVR1B knockout THP-1 cell lysate - PMA treated
1 x 100 µg
Human wild-type THP-1 cell lysate
1 x 100 µg
Human wild-type THP-1 cell lysate - PMA treated
1 x 100 µg

Recommended products

ACVR1B KO cell lysate available now. Free of charge wild type control included.

Key facts

Cell type
THP-1
Disease
Acute Monocytic Leukemia
Concentration
Loading...

Properties

Gene name
ACVR1B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Treatments:
Human ACVR1B knockout THP-1 cell lysate - Untreated
Human wild-type THP-1 cell lysate - Untreated
Human ACVR1B knockout THP-1 cell lysate - PMA (50 ng/mL, 72h)
Human wild-type THP-1 cell lysate - PMA (50 ng/mL, 72h)

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Activin A Receptor Type IB also known as ACVR1B or ALK-4 is a serine/threonine kinase involved in the TGF-beta superfamily signaling. This receptor has a molecular mass of approximately 54 kDa and is expressed in a variety of tissues including the brain heart and reproductive organs. ALK-4 specifically interacts with type II activin receptors to mediate the signal transduction processes that are critical for cell differentiation and growth.

Biological function summary

ALK-4 plays an important role in cell differentiation proliferation and apoptosis. It forms a complex with activin type II receptors which is essential for its proper function. This receptor mediates the signaling pathways that regulate these cellular processes having a significant impact on development and tissue homeostasis. The action of ALK-4 is important for the regulation of mesodermal differentiation and the formation of the axial skeleton during embryogenesis.

Pathways

ALK-4 is part of the TGF-beta signaling pathway and the activin signaling pathway. These pathways are vital for various cellular processes including immune response and tissue repair. In the activin pathway ALK-4 functions along with its related proteins such as ACVR2A and SMAD2/3 to propagate the signal that induces gene expression changes. This signaling cascade involves phosphorylation events that eventually lead to the regulation of target gene expression in the nucleus.

Associated diseases and disorders

ALK-4 has associations with reproductive disorders and cancer. Dysregulation of activin signaling involving ALK-4 can contribute to disorders such as polycystic ovary syndrome (PCOS) and has implications in tumor progression and metastasis in cancer. The receptor works closely with other proteins like inhibin and follistatin which modulate activin signaling highlighting its role in pathologies related to aberrant cell proliferation and differentiation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com