Human ADAM17 knockout K-562 cell lysate

• WB

Lab

Western blot - Human ADAM17 knockout K-562 cell lysate (AB280104)

Lane 1 : Wild-type K562 cell lysate 20 μg
Lane 2 : K562 cell lysate 20 μg
Lane 3 : Raji cell lysate 20 μg
Lane 4 : PC-3 cell lysate 20 μg
False colour image of Western blot : Anti-ADAM17 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to ADAM17. A band was observed at 125 kDa in wild-type K562 cell lysates with no signal observed at this size in ADAM17 knockout cell line ab280045 (knockout cell lysate ab280104). To generate this image, wild-type and ADAM17 knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^®^ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^®^ 800CW) preabsorbed (ab216773) and Goat
anti-Mouse IgG H&L (IRDye^®^ 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Anti-ADAM17 antibody at 1/1000 dilution

Lane 1:

Wild-type K562 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 2:

Western blot - Human ADAM17 knockout K-562 cell line (<a href='/en-us/products/cell-lines/human-adam17-knockout-k-562-cell-line-ab280045'>ab280045</a>)

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

false

• Sanger seq

Unknown

Sanger Sequencing - Human ADAM17 knockout K-562 cell lysate (AB280104)

79 bp deletion in Exon 3.