ADARB1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
Alternative names
1700057H01Rik, ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB 1, AW124433, AW558573, Adenosine deaminase, RNA specific, 2, Adenosine deaminase, RNA specific, B1, Adenosine deaminase, RNA specific, B1 (RED1 homolog rat), Adenosine deaminase, RNA specific, B1 (homolog of rat RED1), BB220382, D10Bwg0447e, DRABA2, DRADA2, Double-stranded RNA-specific editase 1, EC 3.5.-.-, Human dsRNA adenosine deaminase DRADA2, Human dsRNA adenosine deaminase DRADA2b, EC 3.5, OTTHUMP00000115341, OTTHUMP00000115342, RED1_HUMAN, RNA editase, RNA editase 1, RNA editing enzyme 1, rat, homolog of, RNA specific adenosine deaminase B1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
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ADARB1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
- Concentration
Properties
- Gene name
- ADARB1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RED1 also known as ADARB1 is a protein involved in RNA editing. It weighs approximately 73 kDa. RED1 mainly expresses itself in the central nervous system but also appears in other tissues. This protein modifies RNA molecules by catalyzing the conversion of adenosine to inosine which can alter the coding potential and metabolism of the RNA it acts upon.
- Biological function summary
The protein RED1 functions within RNA editing complexes. It co-operates with other proteins like ADAR1 to carry out its editing role. In particular RED1 affects the regulation of gene expression influencing neuronal function and development in the brain. Through its editing capabilities the protein contributes to the diversity and stability of RNA transcripts.
- Pathways
RED1 plays a role in neurotransmission and neurodevelopment pathways. It interacts with other editing proteins in the ADAR family to ensure proper RNA sequence modifications critical for brain function. The editing process that RED1 participates in impacts synaptic transmission important for neuronal communication and neural network formation.
- Associated diseases and disorders
The protein RED1 is associated with neurological disorders. Changes in RED1 expression or mutations can link to conditions such as epilepsy and schizophrenia. Aberrations in RNA editing performed by RED1 might lead to the dysfunction of proteins involved in neuronal signaling. It also connects with proteins like ADAR2 influencing the pathological progress of these conditions.
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1 product image
Sanger Sequencing - Human ADARB1 (RED1) knockout HEK-293T cell lysate (ab258296)
Homozygous: 1 bp insertion in exon5
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