Human ADARB1 (RED1) knockout HeLa cell lysate
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ADARB1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon4.
View Alternative Names
1700057H01Rik, ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB 1, AW124433, AW558573, Adenosine deaminase, RNA specific, 2, Adenosine deaminase, RNA specific, B1, Adenosine deaminase, RNA specific, B1 (RED1 homolog rat), Adenosine deaminase, RNA specific, B1 (homolog of rat RED1), BB220382, D10Bwg0447e, DRABA2, DRADA2, Double-stranded RNA-specific editase 1, EC 3.5.-.-, Human dsRNA adenosine deaminase DRADA2, Human dsRNA adenosine deaminase DRADA2b, EC 3.5, OTTHUMP00000115341, OTTHUMP00000115342, RED1_HUMAN, RNA editase, RNA editase 1, RNA editing enzyme 1, rat, homolog of, RNA specific adenosine deaminase B1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
- Sanger seq
Unknown
Sanger Sequencing - Human ADARB1 (RED1) knockout HeLa cell lysate (AB258295)
Homozygous : 16 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein RED1 functions within RNA editing complexes. It co-operates with other proteins like ADAR1 to carry out its editing role. In particular RED1 affects the regulation of gene expression influencing neuronal function and development in the brain. Through its editing capabilities the protein contributes to the diversity and stability of RNA transcripts.
Pathways
RED1 plays a role in neurotransmission and neurodevelopment pathways. It interacts with other editing proteins in the ADAR family to ensure proper RNA sequence modifications critical for brain function. The editing process that RED1 participates in impacts synaptic transmission important for neuronal communication and neural network formation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB290638
Anti-RED1 antibody [EPR25433-101]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] (AB290638)](https://content.abcam.com/products/images/red1-antibody-epr25433-101-ab290638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175017.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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