Human ADCK3 knockout HEK-293T cell lysate
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COQ8A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon7 and 5 bp deletion in exon7.
View Alternative Names
ADCK3_HUMAN, ARCA2, COQ 8, COQ10D4, COQ8, S. cerevisiae, homolog of, Chaperone ABC1 (activity of bc1 complex S.pombe) like, Chaperone ABC1 activity of bc1 complex homolog, Chaperone activity of bc1 complex like mitochondrial, Chaperone activity of bc1 complex-like, Chaperone-ABC1-like, Coenzyme Q8 homolog, MGC4849, OTTHUMP00000035734, OTTHUMP00000035737, SCAR9, aarF domain-containing protein kinase 3, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human ADCK3 knockout HEK-293T cell lysate (AB263078)
Allele-1 : 5 bp deletion in exon7
- Sanger seq
Unknown
Sanger Sequencing - Human ADCK3 knockout HEK-293T cell lysate (AB263078)
Allele-2 : 2 bp deletion in exon7
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ADCK3 participates in the regulation of coenzyme Q10 levels an essential lipid-soluble antioxidant. The protein is a part of the mitochondrial electron transport chain complex contributing to the production of ATP. As a kinase ADCK3 influences the modulation of the complex's components through phosphorylation stabilizing the function and efficiency of energy conversion processes within mitochondria. Its role is critical for maintaining cellular energy balance and reducing oxidative stress.
Pathways
ADCK3 engages in the coenzyme Q10 biosynthesis pathway. It closely interacts with COQ proteins like COQ9 orchestrating the synthesis and function of the electron transport chain. Additionally it takes part in mitochondrial kinase pathways that ensure proper mitochondrial function. The cooperation between ADCK3 and other proteins such as COQ6 emphasizes its importance in maintaining mitochondrial integrity and energy metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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