Human ADCK4 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
COQ8B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 7 bp deletion in exon 2.
View Alternative Names
ADCK4_HUMAN, Atypical kinase COQ8B mitochondrial, Coenzyme Q8B, NPHS9, Uncharacterized aarF domain-containing protein kinase 4, aarF domain containing protein kinase 4
- Sanger seq
Unknown
Sanger Sequencing - Human ADCK4 knockout HeLa cell lysate (AB263079)
Allele-2 : 1 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human ADCK4 knockout HeLa cell lysate (AB263079)
Allele-1 : 7 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ADCK4 participates in the biosynthesis of coenzyme Q10 a vital component of the electron transport chain. This protein forms part of a larger complex known as the coenzyme Q biosynthesis complex where it helps maintain cellular energy and metabolic balance. It interacts closely with other proteins involved in this pathway to regulate coenzyme Q10 levels which affects multiple cellular processes including ATP production.
Pathways
ADCK4 significantly integrates into the mitochondrial respiratory chain pathway and the broader metabolic energy pathway. It aids in the synthesis of coenzyme Q10 alongside proteins like COQ6 and COQ7 directly impacting the efficiency of energy production in mitochondria. This involvement supports metabolic activities that are critical for cellular homeostasis and energy regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB243641
Anti-KDM3A / JHDM2A antibody [EPR18875-106]
BOND RX™ Validated|RabMAb|Recombinant|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875-106] (AB243641)](https://content.abcam.com/products/images/kdm3a-jhdm2a-antibody-epr18875-106-ab243641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102144.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com