Human ADCY3 (AC3) knockout HeLa cell lysate
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ADCY3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
AC-III, ADCY3_HUMAN, ATP pyrophosphate-lyase, ATP pyrophosphate-lyase 3, Adenylate cyclase, Adenylate cyclase type 3, Adenylate cyclase type III, Adenylyl cyclase 3, adenylate cyclase 3, olfactive type
- Sanger seq
Unknown
Sanger Sequencing - Human ADCY3 (AC3) knockout HeLa cell lysate (AB258297)
Homozygous : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Adenylate cyclase 3 (AC3) serves as an essential component in sensory signal transduction and is part of the adenylyl cyclase complex. It plays an important role in the conversion of external stimuli into intracellular signals important for processes like olfaction and hormonal response. Within neurons AC3 influences synaptic plasticity and learning by regulating cAMP levels. This modulation affects various neural activities and responses making AC3 important for central nervous system functions.
Pathways
AC3 is integrally involved in the G-protein coupled receptor (GPCR) signaling pathways. These pathways involve the activation of AC3 by G-proteins leading to enhanced production of cAMP which subsequently activates protein kinase A (PKA). AC3 collaborates with other adenylate cyclases and G-protein subunits such as Gα and Gβγ within these pathways. This intricate signaling cascade influences cellular responses to hormones and neurotransmitters and alters gene expression through cAMP-mediated pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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