Human ADCY7 knockout HeLa cell lysate
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Human ADCY7 knockout HeLa cell lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
View Alternative Names
ATP pyrophosphate-lyase, Adenylyl cyclase, KIAA0037, adenylate cyclase 7
- Sanger seq
Unknown
Sanger Sequencing - Human ADCY7 knockout HeLa cell lysate (AB263080)
Homozygous : 10 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme participates in the modulation of intracellular signaling pathways by altering cAMP levels. ADCY7 does not usually function alone; it interacts with other signaling molecules forming complexes that influence cellular responses. By regulating cAMP levels ADCY7 can affect processes like cell proliferation differentiation and immune responses.
Pathways
Adenylate cyclase activity of ADCY7 plays a significant role in G protein-coupled receptor (GPCR) signaling and is involved in the downstream cAMP signaling pathway. These pathways are critical for transmitting signals from extracellular stimuli to intracellular effectors. ADCY7 interacts with G proteins which directly influence its activity. It is also associated with pathways including the MAPK signaling involving a chain of proteins in the cell that communicates signals from receptors on the cell surface to the DNA in the nucleus.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product protocols
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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