Human ADK knockout HeLa cell lysate
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ADK KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon2 and 41 bp deletion in exon2.
View Alternative Names
2310026J05Rik, 5033405D03Rik, ADK_HUMAN, AI255373, AI987814, AK, Adenosine 5''-phosphotransferase, Adenosine kinase, MGC6593, OTTHUMP00000019864, OTTHUMP00000019865
- WB
Lab
Western blot - Human ADK knockout HeLa cell lysate (AB257343)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : ADK knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab243636 observed at 41 kDa. Red - loading control ab8245 observed at 37 kDa.
ab243636 Anti-ADK antibody [EPR23166-143] was shown to specifically react with Adenosine kinase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265646 (knockout cell lysate ab257343) was used. Wild-type and Adenosine kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab243636 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ADK antibody [EPR23166-143] (<a href='/en-us/products/primary-antibodies/adk-antibody-epr23166-143-ab243636'>ab243636</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ADK knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ADK knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-adk-knockout-hela-cell-line-ab265646'>ab265646</a>)
Predicted band size: 41 kDa
Observed band size: 41 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ADK knockout HeLa cell lysate (AB257343)
Allele-2 : 13 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ADK knockout HeLa cell lysate (AB257343)
Allele-1 : 41 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.
Pathways
The activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB243636
Anti-ADK antibody [EPR23166-143]
BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size|Trial Size|Trial Size
![Western blot - Anti-ADK antibody [EPR23166-143] (AB243636)](https://content.abcam.com/products/images/adk-antibody-epr23166-143-ab243636--western-blot-img55323.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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