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AB257345

Human ADRBK1 (GRK2) knockout HEK-293T cell lysate

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GRK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
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Western blot - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)
  • WB

Unknown

Western blot - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ADRBK1 knockout HEK293T cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab227825 was shown to specifically react with GRK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266352 (knockout cell lysate ab257345) was used. Wild-type and GRK2 knockout samples were subjected to SDS-PAGE. ab227825 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)
  • Sanger seq

Unknown

Sanger Sequencing - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)

Allele-1 : 1 bp insertion in exon 2

Sanger Sequencing - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)
  • Sanger seq

Unknown

Sanger Sequencing - Human ADRBK1 (GRK2) knockout HEK-293T cell lysate (AB257345)

Allele-2 : Insertion of the selection cassette in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.

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Complementary Products

Primary Antibodies

AB227825

Anti-GRK2 antibody [EPR22465]

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-GRK2 antibody [EPR22465] (AB227825)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Proteins & Peptides

AB180317

Recombinant Human POLR1C protein

SDS-PAGE - Recombinant Human POLR1C protein (AB180317)

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Proteins & Peptides

AB125620

Recombinant human GRK2 protein

Functional Studies - Recombinant human GRK2 protein (AB125620)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
GRK2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GRK2 also known as G protein-coupled receptor kinase 2 is an important enzyme involved in the regulation of G protein-coupled receptors (GPCRs). It has a molecular weight of approximately 80 kilodaltons. GRK2 is widely expressed in many tissues especially in the heart and brain where it plays an important role in cellular signaling processes. Its primary action is phosphorylating activated GPCRs which leads to receptor desensitization and downregulation modulating cellular responses to stimuli.
Biological function summary

GRK2 acts by coordinating with other proteins to regulate signal transduction pathways. It usually forms a complex with arrestins which prevent further signaling by blocking G protein coupling and directing receptors towards internalization pathways. This kinase acts to modulate receptor activity and maintain cellular homeostasis. GRK2 is essential for preventing overstimulation of cells playing a role in neurobiology and cardiology.

Pathways

GRK2 plays an important role in the adrenergic receptor signaling pathways including the beta-adrenergic and alpha-adrenergic pathways. It participates in the regulation of cardiac output and vascular tone by influencing the response of cells to catecholamines like adrenaline and noradrenaline. Proteins related to GRK2 in these pathways include adrenergic receptor subtypes and arrestin proteins. Voltage-gated calcium channels also interact with GRK2 linking it to calcium signaling pathways vital for muscle contraction and neurotransmitter release.

Research highlights GRK2's involvement in heart failure and hypertension. In heart failure elevated levels of GRK2 correlate with worsened cardiac function due to impaired beta-adrenergic receptor signaling. GRK2 is also linked to the development of hypertension through its effects on vascular smooth muscle cells and blood pressure regulation. Its interaction with proteins like arrestins suggests potential therapeutic targets for these cardiovascular conditions. Understanding and modulating GRK2 activity might offer approaches to treat these disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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