Human AGA knockout HEK-293T cell lysate
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AGA KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
View Alternative Names
AGU, ASPG_HUMAN, ASRG, Aspartylglucosaminidase, Aspartylglucosylamine deaspartylase, Aspartylglycosaminuria, GA, Glycosylasparaginase, Glycosylasparaginase beta chain, N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase, N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
- Sanger seq
Unknown
Sanger Sequencing - Human AGA knockout HEK-293T cell lysate (AB263081)
Homozygous : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Enzymes like AGA help in glycoprotein catabolism which is an essential process for cellular maintenance and function. AGA functions as a homodimer and is an integral part of the lysosomal enzyme complex. This complex ensures proper degradation of N-linked oligosaccharides from glycoproteins following cellular internalization. Disruption in AGA's function results in accumulation of undigested glycoproteins which may affect normal cellular processes.
Pathways
AGA's activity ties into the lysosomal degradation pathway significantly influencing the catabolic reduction of glycoproteins. It is closely related to other lysosomal enzymes such as cathepsins. AGA’s regulation is linked to cellular recycling and energy balance pathways transitioning the breakdown products of glycoproteins into reusable components for other metabolic processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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