Human AGO3 knockout HeLa cell lysate
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- WB
Lab
Western blot - Human AGO3 knockout HeLa cell lysate (AB257819)
Lane 1 : Wild-type HeLa cell lysate (40μg)
Lane 2 : Argonaute RISC Catalytic Component 3 knockout HeLa cell lysate (40μg)
Lane 3 : Daudi cell lysate (40μg)
Lane 4 : A549 cell lysate (40μg)
Lanes 1-4 : Merged signal (red and green). Green - ab154844 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.
ab154844 Anti-AGO3 antibody [EPR9576] was shown to specifically react with Argonaute RISC Catalytic Component 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265320 (knockout cell lysate ab257819) was used. Wild-type and Argonaute RISC Catalytic Component 3 knockout samples were subjected to SDS-PAGE. ab154844 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lanes 1 - 4:
Western blot - Anti-AGO3 antibody [EPR9576] (<a href='/en-us/products/primary-antibodies/ago3-antibody-epr9576-ab154844'>ab154844</a>) at 1/2000 dilution
Lane 3:
154844 at 1/2000 dilution
Lane 1:
Wild-type HeLa lysate at 40 µg
Lane 2:
Argonaute RISC Catalytic Component 3 knockout HeLa lysate at 40 µg
Lane 2:
Western blot - Human AGO3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ago3-knockout-hela-cell-line-ab265320'>ab265320</a>)
Lane 3:
Daudi lysate at 40 µg
Lane 4:
A549 lysate at 40 µg
Predicted band size: 97 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human AGO3 knockout HeLa cell lysate (AB257819)
Homozygous : 14 bp deletion in exon12
Complementary Products
![Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)](https://content.abcam.com/products/images/ago3-antibody-epr9576-ab154844--western-blot-img143878.jpg)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AGO3 contributes to gene expression regulation by silencing specific RNA transcripts. This protein forms a complex with other proteins and RNA molecules to execute its functions. Part of the miRNA and RNA interference pathways AGO3 associates with Dicer and other Argonaute proteins creating an effective silencing machinery that modulates various cellular processes. Its activity is essential for maintaining cellular homeostasis through RNA interference-mediated gene silencing.
Pathways
AGO3 plays a significant role in the RNA interference (RNAi) and the microRNA (miRNA) pathways. These pathways are key in post-transcriptional gene regulation controlling gene expression patterns important for development and differentiation. AGO3 interacts with Dicer a pivotal enzyme in the RNAi pathway facilitating the processing of precursor miRNAs into mature miRNAs further guiding RISC to its target mRNA sequences. These interactions highlight AGO3's critical place in RNA silencing networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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