Human AIP knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
AIP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
AH receptor-interacting protein, AIP_HUMAN, ARA 9, Aryl-hydrocarbon receptor-interacting protein, FKBP 16, FKBP 37, HBV X associated protein, HBV X-associated protein 2, Immunophilin homolog ARA 9, SMTPHN, XAP-2, fa03h10
- WB
Lab
Western blot - Human AIP knockout HeLa cell lysate (AB257822)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : AIP knockout HeLa cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.
ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-AIP antibody [EPR13585] (<a href='/en-us/products/primary-antibodies/aip-antibody-epr13585-ab192024'>ab192024</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
AIP knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human AIP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-aip-knockout-hela-cell-line-ab265502'>ab265502</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human AIP knockout HeLa cell lysate (AB257822)
Homozygous : 1 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AIP acts as a modulator of protein-protein interactions impacting several signaling pathways. It is a part of the larger protein complex involving heat shock proteins which helps prevent the degradation of its client proteins. AIP is essential for the activity and stability of receptors like the aryl hydrocarbon receptor (AHR) helping these receptors remain in a functional state until they get activated.
Pathways
AIP incorporates itself into the AHR signaling pathway which affects the regulation of xenobiotic metabolism. The protein is also involved in the regulation of the glucocorticoid receptor pathway working closely with proteins like Hsp90. Through these interactions AIP influences the ability of cells to respond to environmental toxins and physiological stress thereby playing a role in cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-AIP antibody [EPR13585] (AB192024)](https://content.abcam.com/products/images/aip-antibody-epr13585-ab192024--western-blot-img4774.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com