Human AK2 knockout HEK-293T cell lysate
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AK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 8 bp deletion in exon 1.
View Alternative Names
ADK2, ATP-AMP transphosphorylase, ATP-AMP transphosphorylase 2, Adenylate kinase 2, Adenylate kinase isoenzyme 2, EC 2.7.4.3, KAD2_HUMAN, adenylate kinase 2, mitochondrial, mitochondrial
- WB
Unknown
Western blot - Human AK2 knockout HEK-293T cell lysate (AB257825)
Lane 1 : Wild-type HEK-293T cell lysate 20 ug
Lane 2 : AK2 knockout HEK-293T cell lysate 20 ug
Lane 3 : HL-60 cell lysate 20 ug
Lane 4 : HepG2 cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab166901 observed at 26 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab166901 was shown to react with AK2 in wild-type HEK-293T cells in western blot with loss of signal observed in AK2 knockout cell line ab266539 (AK2 knockout cell lysate ab257825). HEK-293T wild-type and AK2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab166901 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-AK2 antibody [EPR11388(B)] (<a href='/en-us/products/primary-antibodies/ak2-antibody-epr11388b-ab166901'>ab166901</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
AK2 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human AK2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ak2-knockout-hek-293t-cell-line-ab266539'>ab266539</a>)
Lane 3:
HL-60 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Predicted band size: 26 kDa
Observed band size: 26 kDa
false
- WB
Lab
Western blot - Human AK2 knockout HEK-293T cell lysate (AB257825)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : AK2 knockout HEK293T cell lysate (20 ug)
Lane 3 : HL-60 cell lysate (20 ug)
Lane 4 : HepG2 cell lysate (20 ug)
ab157206 was shown to specifically react with AK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266539 (knockout cell lysate ab257825) was used. Wild-type and AK2 knockout samples were subjected to SDS-PAGE. ab157206 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-AK2 antibody [EPR11387(B)] (<a href='/en-us/products/primary-antibodies/ak2-antibody-epr11387b-ab157206'>ab157206</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
AK2 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human AK2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ak2-knockout-hek-293t-cell-line-ab266539'>ab266539</a>)
Lane 3:
HL-60 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human AK2 knockout HEK-293T cell lysate (AB257825)
Allele-1 : 8 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human AK2 knockout HEK-293T cell lysate (AB257825)
Allele-2 : 1 bp insertion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AK2 functions in energy transfer and regulation in the cell serving as an important component in maintaining cellular ATP levels. AK2 is an integral part of the mitochondrial adenylate kinase system which manages energy flux in cells. By balancing the concentrations of ATP ADP and AMP AK2 directly influences cellular energy state and metabolic activity. The enzyme also supports nucleotide homeostasis therefore facilitating processes such as signal transduction and nucleic acid synthesis.
Pathways
AK2 is involved in the adenylate kinase pathway important for energy metabolism. It coordinates with other enzymes like AK1 and AK3 ensuring efficient energy distribution in the cell. AK2 also interfaces with the apoptosis signaling pathways where it plays a part in regulating programmed cell death. The interaction of AK2 within these pathways emphasizes its essential role in maintaining cellular energy balance and undergoing apoptosis when necessary.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB249383
Anti-AK2 antibody [EPR11388(B)] - BSA and Azide free
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] - BSA and Azide free (AB249383)](https://content.abcam.com/products/images/ak2-antibody-epr11388b-bsa-and-azide-free-ab249383--immunocytochemistry-immunofluorescence-img77411.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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