AK3 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 7 bp deletion in exon2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 7 bp deletion in exon2.
Alternative names
AK3, formerly, AK4, AK4, mouse, homolog of, AKL3L, AKL3L1, Adenylate kinase 3, Adenylate kinase 3 alpha-like 1, Adenylate kinase 3, formerly, FIX, GTP:AMP phosphotransferase, GTP:AMP phosphotransferase, mitochondrial, KAD3_HUMAN, OTTHUMP00000021015, OTTHUMP00000021016, RP11 6J24.4, adenylate kinase 6, adenylate kinase 3 like 1, mitochondrial
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AK3 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 7 bp deletion in exon2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 7 bp deletion in exon2.
- Concentration
Properties
- Gene name
- AK3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AK3 also known as adenylate kinase 3 is a mitochondrial enzyme involved in energy metabolism. It has a molecular mass of approximately 26 kDa and is mainly found in the mitochondrial matrix. AK3 catalyzes the reversible transfer of phosphate groups among adenosine phosphates which plays a role in cellular energy homeostasis. Expression of AK3 occurs in tissues with high metabolic rates such as the heart liver and muscle reflecting its importance in energy demand areas.
- Biological function summary
Adenylate kinase 3 facilitates the maintenance of cellular energy balance by ensuring the proper concentration of adenine nucleotides within the mitochondria. This enzyme is an important component of the adenine nucleotide translocase complex which regulates nucleotide transport across the inner mitochondrial membrane. Through its activity AK3 contributes to the recycling of ATP and ADP supporting overall energy metabolism and mitochondrial function.
- Pathways
Adenylate kinase 3 plays a significant role in the purine nucleotide cycle and oxidative phosphorylation pathways. In the purine nucleotide cycle it interacts with enzymes like nucleoside diphosphate kinase for maintaining proper levels of ADP and ATP. Within oxidative phosphorylation AK3 helps in balancing energy supply in concert with other mitochondrial proteins like ATP synthase ensuring efficient ATP production and utilization in energy-intensive processes.
- Associated diseases and disorders
Alterations in adenylate kinase 3 function have been linked to mitochondrial diseases and cardiac disorders. Mitochondrial dysfunction resulting from impaired AK3 activity can lead to energy deficiency contributing to diseases like Leigh syndrome. Similarly inadequate AK3 function may affect cardiac energy metabolism relating to heart failure conditions where proper energy supply is essential. AK3's interactions with proteins like creatine kinase further emphasize its role in maintaining cardiac energy stability.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human AK3 knockout HEK-293T cell lysate (ab257826)
Allele-1: 7 bp deletion in exon2
Sanger Sequencing - Human AK3 knockout HEK-293T cell lysate (ab257826)
Allele-2: 1 bp deletion in exon2
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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