Human AK4 (AK3L1) knockout HeLa cell lysate
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- WB
Lab
Western blot - Human AK4 (AK3L1) knockout HeLa cell lysate (AB257827)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : AK4 knockout HeLa cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab131327 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.
ab131327 Anti-AK3L1 antibody [EPR7678] was shown to specifically react with Adenylate kinase 4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265331 (knockout cell lysate ab257827) was used. Wild-type and Adenylate kinase 4 knockout samples were subjected to SDS-PAGE. ab131327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-AK3L1 antibody [EPR7678] (<a href='/en-us/products/primary-antibodies/ak3l1-antibody-epr7678-ab131327'>ab131327</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
AK3L1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human AK4 (AK3L1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ak4-ak3l1-knockout-hela-cell-line-ab265331'>ab265331</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human AK4 (AK3L1) knockout HeLa cell lysate (AB257827)
Homozygous : 2 bp insertion in exon2
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3L1 antibody [EPR7678] (AB131327)](https://content.abcam.com/products/images/ak3l1-antibody-epr7678-ab131327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121139.jpg)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AK3L1 regulates mitochondrial energy metabolism and supports cellular functions. It operates as part of a network managing the mitochondrial adenylate kinase isoform family participating in mitochondrial phosphotransfer systems. AK3L1 contributes to sustaining energy balance especially in tissues with high-energy demand like muscle and brain. Within these systems AK3L1 interacts with other adenylate kinases and mitochondrial proteins to balance nucleotide ratios and prevent energy deficits.
Pathways
AK3L1 integrates into the wider cellular energy regulation and oxidative phosphorylation pathways. It supports the cellular energy landscape by linking to the citric acid cycle and oxidative phosphorylation essential for ATP production. AK3L1 coordinates with proteins like AK2 influencing energy metabolism and ensuring effective cellular processes. By aligning with these pathways AK3L1 plays a significant role in ensuring efficient bioenergetic processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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