Human AKAP1 knockout HEK-293T cell lysate
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AKAP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
A kinase anchor protein, A-kinase anchor protein 1, A-kinase anchor protein 149 kDa, AKAP, AKAP 149, AKAP1_HUMAN, D-AKAP-1, Dual specificity A-kinase-anchoring protein 1, PRKA1, Protein kinase A-anchoring protein 1, Protein kinase A1, S-AKAP84, Spermatid A-kinase anchor protein 84, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human AKAP1 knockout HEK-293T cell lysate (AB257828)
Allele-2 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human AKAP1 knockout HEK-293T cell lysate (AB257828)
Allele-1 : 11 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AKAP1 connects with several components to influence cellular signaling pathways and energy metabolism. It is a part of a complex network that involves mitochondrial targeting sequences which guide proteins to correct mitochondrial compartments. AKAP1 helps regulate processes like mitochondrial dynamics apoptosis and oxidative phosphorylation. These functions ensure efficient cellular energy production and maintenance of cellular health.
Pathways
AKAP1 engages in both the cAMP-dependent and mitochondrial signaling pathways. In the cAMP-dependent pathway AKAP1 interacts with PKA to modulate phosphorylation events that alter cellular responses to external signals. The mitochondrial pathway involves communication with proteins like Drp1 influencing mitochondrial fission and fusion processes. These interactions highlight AKAP1's central role in maintaining cellular energy balance and integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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