Human AKAP11 (AKAP 220) knockout HeLa cell lysate
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AKAP11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 26 bp deletion in exon8.
View Alternative Names
A-kinase anchor protein 11, A-kinase anchor protein 220 kDa, AKA11_HUMAN, AKAP 220, AKAP-11, PRKA11, Protein kinase A-anchoring protein 11, hAKAP220
- Sanger seq
Unknown
Sanger Sequencing - Human AKAP11 (AKAP 220) knockout HeLa cell lysate (AB263082)
Allele-2 : 1 bp deletion in exon8
- Sanger seq
Unknown
Sanger Sequencing - Human AKAP11 (AKAP 220) knockout HeLa cell lysate (AB263082)
Allele-1 : 26 bp deletion in exon8
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AKAP 220 anchors PKA and other signaling molecules to form a multi-protein complex. This complex facilitates the spatial and temporal regulation of PKA activity allowing cells to respond accurately to changes in cyclic AMP levels. AKAP 220 also interacts with other proteins such as phosphatases and cytoskeletal elements contributing to its role in modulating various cellular processes. It controls signal transduction pathways important for neuronal communication and reproduction due to its complex-forming ability.
Pathways
The protein plays an important role in the cAMP signaling and MAPK/ERK pathways. In these pathways AKAP 220 coordinates PKA with components like Ras and Raf essential players in cellular responses such as growth division and differentiation. By linking PKA to precise signaling cascades AKAP 220 influences the modulation of cellular processes that these pathways regulate impacting how cells react to external signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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