Human ALDH18A1 (P5CS) knockout HEK-293T cell lysate
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ALDH18A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
View Alternative Names
2810433K04Rik, AI429789, ALDH18A1, Aldehyde dehydrogenase 18 family member A1, Aldehyde dehydrogenase 18A1, Aldehyde dehydrogenase family 18 member A1, Delta 1 pyrroline 5 carboxylate synthetase, Delta1 pyrroline 5 carboxlate synthetase, GK, GPR, GSAS, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase, Glutamate-5-semialdehyde dehydrogenase, Glutamyl-gamma-semialdehyde dehydrogenase, MGC117316, MGC32233, P5CS_HUMAN, PYCS, Pyrroline 5 carboxylate synthetase (glutamate gamma semialdehyde synthetase)
- Sanger seq
Unknown
Sanger Sequencing - Human ALDH18A1 (P5CS) knockout HEK-293T cell lysate (AB258300)
Allele-1 : 1 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human ALDH18A1 (P5CS) knockout HEK-293T cell lysate (AB258300)
Allele-2 : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P5CS influences cellular responses to oxidative stress due to its role in proline metabolism and redox balance. As part of the proline synthesis pathway P5CS contributes to the production of proline a critical amino acid for protein synthesis. Proline acts as an osmoprotectant and plays a role in cellular structure. P5CS operates within a complex pathway regulating amino acids acting cooperatively with other proteins involved in synthesis processes.
Pathways
P5CS is integral to the proline biosynthetic pathway and is linked to the glutamate pathway. Its activity closely coordinates with the functioning of enzymes like pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline. This relationship ensures the continuous supply of proline vital for collagen synthesis and also intersects with pathways involving protein metabolism and cellular stress responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB315817
Anti-P5CS antibody [EPR28827-56] - BSA and Azide free
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody [EPR28827-56] - BSA and Azide free (AB315817)](https://content.abcam.com/products/images/p5cs-antibody-epr28827-56-bsa-and-azide-free-ab315817--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425470.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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