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AB270506

Human ALDH2 knockout SW480 cell lysate

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ALDH2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift: 99.83%.
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Western blot - Human ALDH2 knockout SW480 cell lysate (AB270506)
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Western blot - Human ALDH2 knockout SW480 cell lysate (AB270506)

Lane 1 : Wild-type SW480 cell lysate 20 ug

Lane 2 : ALDH2 knockout SW480 cell lysate 20 ug

Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot Loss of signal was observed when ALDH2 knockout cell line ab270483 (knockout cell lysate ab270506) was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ALDH2 antibody [EPR4494] (<a href='/en-us/products/primary-antibodies/aldh2-antibody-epr4494-ab133306'>ab133306</a>) at 1/1000 dilution

Lane 1:

Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 56 kDa

Observed band size: 53 kDa,56 kDa

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Key facts

Cell type

SW480

Species or organism

Human

Tissue

Colon

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift: 99.83%

Disease

Adenocarcinoma

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Complementary Products

Proteins & Peptides

AB87415

Recombinant human ALDH2 protein (Active) (Tag Free)

Bioactive

SDS-PAGE - Recombinant human ALDH2 protein (Active) (Tag Free) (AB87415)

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Primary Antibodies

AB133306

Anti-ALDH2 antibody [EPR4494]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-ALDH2 antibody [EPR4494] (AB133306)

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Biochemicals

AB146598

Trichostatin A (DMSO solution), histone deacetylase inhibitor

Chemical Structure - Trichostatin A (DMSO solution), histone deacetylase inhibitor (AB146598)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ALDH2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH2 also known as aldehyde dehydrogenase 2 or ADHL2 is a mitochondrial enzyme with a mass of approximately 56 kDa. It resides mainly in the liver although other tissues exhibit expression as well. ALDH2 plays a critical role in metabolizing acetaldehyde a toxic byproduct of alcohol metabolism by converting it into acetate which is less harmful. This process protects cells from the damaging effects of acetaldehyde accumulation.
Biological function summary

ALDH2 serves a detoxifying function by preventing oxidative stress and promoting cellular health. As part of the larger aldehyde dehydrogenase family this enzyme forms homotetramer complexes to efficiently catalyze the oxidation of aldehydes. ALDH2 supplementation may enhance its activity in individuals with reduced function due to genetic polymorphisms providing potential therapeutic benefits.

Pathways

ALDH2 integrates into the alcohol metabolism and retinoic acid signaling pathways. In alcohol metabolism it collaborates with alcohol dehydrogenase enzymes such as ADH1 and ADH2 to process alcohol consumption compounds. In retinoic acid signaling ALDH2 helps regulate the biosynthesis of retinoic acid influencing cell proliferation and differentiation processes.

ALDH2 activity impacts conditions like alcohol flush reaction and cardiovascular diseases. Individuals with ALDH2 deficiency often due to genetic variations like ALDH2*2 experience decreased acetaldehyde clearance leading to flush reactions and an increased risk for certain cancers. Additionally inadequate ALDH2 function relates to elevated oxidative stress contributing to cardiovascular disease risk where proteins such as MSD161 may have interactions relevant for therapeutic targeting.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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