Human ANGPTL6 knockout A549 cell lysate
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ANGPTL6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon3 and 5 bp deletion in exon3.
View Alternative Names
AGF, ANGL6_HUMAN, Angiopoietin like 6, Angiopoietin-like protein 6, Angiopoietin-related growth factor, Angiopoietin-related protein 5, Angiopoietin-related protein 6
- Sanger seq
Unknown
Sanger Sequencing - Human ANGPTL6 knockout A549 cell lysate (AB263085)
Allele-2 : 4 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human ANGPTL6 knockout A549 cell lysate (AB263085)
Allele-1 : 5 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ANGPTL6 functions in the regulation of metabolic processes including glucose and lipid metabolism. It is not part of a larger complex but acts individually in its biological roles. ANGPTL6 has been shown to improve insulin sensitivity and promote energy expenditure. Through these mechanisms it plays an essential role in maintaining metabolic homeostasis and supports cellular health.
Pathways
ANGPTL6 significantly interacts within metabolic and cardiovascular pathways. The protein influences the insulin signaling pathway and is connected to proteins like AKT and PI3K integral components of metabolic regulation. ANGPTL6 also affects pathways involved in angiogenesis providing cross-talk with vascular endothelial growth factor (VEGF)-mediated signaling an important aspect of cardiovascular health.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB281241
Anti-ANGPTL6 antibody [EPR23076-12] - BSA and Azide free (Capture)
RabMAb|Recombinant
![Sandwich ELISA - Anti-ANGPTL6 antibody [EPR23076-12] - BSA and Azide free (Capture) (AB281241)](https://content.abcam.com/products/images/angptl6-antibody-epr23076-12-bsa-and-azide-free-capture-ab281241--sandwich-elisa-img429539.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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