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AB263086

Human ANKRD13A knockout HeLa cell lysate

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ANKRD13A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1.
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Sanger Sequencing - Human ANKRD13A knockout HeLa cell lysate (AB263086)
  • Sanger seq

Unknown

Sanger Sequencing - Human ANKRD13A knockout HeLa cell lysate (AB263086)

Homozygous : 7 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265254

Human ANKRD13A knockout HeLa cell line

Sanger Sequencing - Human ANKRD13A knockout HeLa cell line (AB265254)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ANKRD13A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD13A also known as Ankyrin Repeat Domain 13A is a protein weighing approximately 114 kDa. It contains ankyrin repeat motifs that facilitate protein-protein interactions making it important for cellular processes. ANKRD13A expresses widely across tissues with the highest levels in the brain heart and skeletal muscle. These repeat motifs are characteristic of proteins that participate in a variety of cellular functions suggesting ANKRD13A's role in maintaining cellular architecture and signaling.
Biological function summary

ANKRD13A interacts with several proteins implicating it in cellular adhesion and signaling pathways. It forms part of a complex involved in endocytosis and intracellular trafficking. These processes are essential for maintaining cellular communication and signaling balance. ANKRD13A's protein interactions suggest functions in regulating cell membrane dynamics and vesicle transport which are critical for constant cellular adaptation to environmental signals.

Pathways

ANKRD13A plays a significant role in endocytosis and neurotransmitter trafficking. It interacts within the endocytic pathway to regulate the internalization and sorting of membrane proteins. ANKRD13A has connections to clathrin-mediated endocytosis and may influence synaptic vesicle recycling. The protein associates with clathrin and adaptor protein complexes indicating its contribution to maintaining neurotransmitter homeostasis in neural pathways.

ANKRD13A has associations with neurological and cardiovascular conditions. Deregulation or mutations in ANKRD13A may contribute to neurodegenerative diseases such as Alzheimer's and heart diseases. The protein's link to dynamin and other cytoskeletal proteins is critical since these proteins play roles in amyloid precursor protein (APP) processing and endocytic pathways related to these illnesses. Understanding how ANKRD13A interacts in these contexts can help in exploring therapeutic options for these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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