Human ANP32A (PHAP1) knockout HEK-293T cell lysate
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ANP32A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1.
View Alternative Names
AN32A_HUMAN, ANP32A, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, C15orf1, Cerebellar leucine rich acidic nuclear protein, HPPCn, Hepatopoietin Cn, I1PP2A, Lanp, Leucine-rich acidic nuclear protein, MAPM, MGC119787, MGC150373, Mapmodulin, PHAPI, PP32, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, Putative human HLA class II associated protein I, Putative human HLA class II-associated protein, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, inhibitor-1 of protein phosphatase-2A
- WB
Lab
Western blot - Human ANP32A (PHAP1) knockout HEK-293T cell lysate (AB258303)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : ANP32A knockout HEK-293T cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : THP-1 cell lysate 20 μg
False colour image of Western blot : Anti-PHAP1 antibody - C-terminal staining at 0.5 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab189110 was shown to bind specifically to PHAP1. A band was observed at 33 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in ANP32A knockout cell line ab266148 (knockout cell lysate ab258303). To generate this image, wild-type and ANP32A knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-PHAP1 antibody - C-terminal (<a href='/en-us/products/primary-antibodies/phap1-antibody-c-terminal-ab189110'>ab189110</a>) at 0.5 µg/mL
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
ANP32A knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human ANP32A (PHAP1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-anp32a-phap1-knockout-hek-293t-cell-line-ab266148'>ab266148</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
THP-1 cell lysate at 20 µg
Predicted band size: 28 kDa
Observed band size: 33 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ANP32A (PHAP1) knockout HEK-293T cell lysate (AB258303)
Homozygous : 8 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PHAP1 plays an important role in regulating apoptosis and cell proliferation. It interacts with components of the INHAT complex which inhibits histone acetylation impacting chromatin remodeling and gene expression. By doing so it influences many cellular events such as cell cycle progression and differentiation. PHAP1's involvement in regulating cell death makes it a target for studying mechanisms underlying cell survival.
Pathways
PHAP1 is associated with apoptosis and cell signaling processes. It is intricately linked with the p38 MAPK signaling pathway important in stress response and inflammation. In these contexts PHAP1 interacts with proteins such as caspases and protein phosphatase 2A which are important for cellular responses to external and internal stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266148
Human ANP32A (PHAP1) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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