Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell lysate
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ANP32B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon1.
View Alternative Names
AN32B_HUMAN, ANP 32B, APRIL, Acidic (leucine rich) nuclear phosphoprotein 32 family member B, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, OTTHUMP0000006377, PAL 31, PHAPI 2, PHAPI2 protein, PHAPI2a, Proliferation related acidic leucine rich protein, Putative HLA-DR-associated protein I-2, Silver stainable protein SSP 29, Silver-stainable protein SSP29, Ssp 29
- WB
Lab
Western blot - Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell lysate (AB257831)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ANP32B knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : Lncap cell lysate (20 ug)
ab200836 was shown to specifically react with PHAPI2 / APRIL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266818 (knockout cell lysate ab257831) was used. Wild-type and PHAPI2 / APRIL knockout samples were subjected to SDS-PAGE. ab200836 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PHAPI2 / APRIL antibody [EPR14588] (<a href='/en-us/products/primary-antibodies/phapi2-april-antibody-epr14588-ab200836'>ab200836</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-anp32b-phapi2-april-knockout-hek-293t-cell-line-ab266818'>ab266818</a>)
Lane 2:
ANP32B knockout HEK293T cell lysate at 20 µg
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
LNCaP cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell lysate (AB257831)
Homozygous : 13 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PHAPI2 regulates immune system processes by playing a role in B cell development and activity. It functions as part of larger protein complexes binding with proteins such as BCMA to influence signaling pathways. The interactions within these complexes help modulate processes critical for proper immune response and maintenance of immune system homeostasis.
Pathways
PHAPI2 is deeply involved in both the NF-kB signaling pathway and the PI3K/AKT pathway. These pathways are essential for cellular growth survival and proliferation. Through its involvement PHAPI2 also connects with proteins like NF-kB and AKT1 which are key regulatory proteins within these pathways. This connection enables PHAPI2 to act as a node integrating signals for various cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB200836
Anti-PHAPI2 / APRIL antibody [EPR14588]
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-PHAPI2 / APRIL antibody [EPR14588] (AB200836)](https://content.abcam.com/products/images/phapi2-april-antibody-epr14588-ab200836--immunocytochemistry-immunofluorescence-img14994.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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