ANTXR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 322 bp insertion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 322 bp insertion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Alternative names
ANTR1_HUMAN, Anthrax toxin receptor 1, Antxr1, Tumor endothelial marker 8
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ANTXR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 322 bp insertion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 322 bp insertion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- ANTXR1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (ab257350)
Lane 1: Wild-type HeLa cell lysate 20 μg
Lane 2: ANTXR1 knockout HeLa cell lysate 20 μg
Lane 3: SW480 cell lysate 20 μg
Lane 4: U-2 OS cell lysate 20 μg
False colour image of Western blot: Anti-TEM8/ATR antibody staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-TEM8/ATR antibody ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line Human ANTXR1 (TEM8/ATR) knockout HeLa cell line ab265077 (knockout cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-TEM8/ATR antibody (Anti-TEM8/ATR antibody ab21269) at 1 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell line (Human ANTXR1 (TEM8/ATR) knockout HeLa cell line ab265077)
Lane 2: ANTXR1 knockout HeLa cell lysate at 20 µg
Lane 3: SW480 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (ab257350)
Allele-2: 4 bp deletion in exon1
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (ab257350)
Allele-3: Insertion of the selection cassette in exon1
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (ab257350)
Allele-1: 322 bp insertion in exon1
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