Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate
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- WB
Supplier Data
Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (AB257350)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ANTXR1 knockout HeLa cell lysate 20 μg
Lane 3 : SW480 cell lysate 20 μg
Lane 4 : U-2 OS cell lysate 20 μg
False colour image of Western blot : Anti-TEM8/ATR antibody staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line ab265077 (knockout cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-TEM8/ATR antibody (<a href='/en-us/products/primary-antibodies/tem8-atr-antibody-ab21269'>ab21269</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-antxr1-tem8-atr-knockout-hela-cell-line-ab265077'>ab265077</a>)
Lane 2:
ANTXR1 knockout HeLa cell lysate at 20 µg
Lane 3:
SW480 cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Predicted band size: 63 kDa
Observed band size: 75 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (AB257350)
Allele-3 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (AB257350)
Allele-2 : 4 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate (AB257350)
Allele-1 : 322 bp insertion in exon1
Complementary Products
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Cell Lines & Lysates
AB265077
Human ANTXR1 (TEM8/ATR) knockout HeLa cell line
Advanced Validation
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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