Human ANXA11 (Annexin-11) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
ANXA11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
View Alternative Names
56 kDa autoantigen, ANX A11, ANX11_HUMAN, Annexin A11, Annexin XI, Annexin-11, Autoantigen 56 kD, CAP-50, Calcyclin-associated annexin 50, OTTHUMP00000059806
- Sanger seq
Unknown
Sanger Sequencing - Human ANXA11 (Annexin-11) knockout HEK-293T cell lysate (AB258782)
Homozygous : 1 bp insertion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Annexin A11 plays a significant role in cellular processes such as membrane repair cell migration and apoptosis. It does not form a stable part of a large protein complex under normal conditions but interacts dynamically with other proteins during cellular events. This protein influences intracellular calcium signaling which is important for maintaining cellular homeostasis and responding to extracellular stimuli. Annexin A11 regulates actin cytoskeletal organization impacting cell motility and structural integrity.
Pathways
Annexin A11’s functions integrate into critical cell signaling and communication pathways such as the calcium signaling pathway and apoptosis pathway. Within these pathways annexin A11 interacts with other proteins like annexin V and annexin II modifying intracellular responses to calcium fluctuations and apoptotic signals. These pathways ensure proper cellular responses to environmental changes and stress conditions facilitating processes like cell survival and programmed cell death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB300577
Anti-Annexin-11/ANXA11 antibody [EPR26004-63]
20ul selling size|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)](https://content.abcam.com/products/images/annexin-11-anxa11-antibody-epr26004-63-ab300577--immunocytochemistry-immunofluorescence-img400723.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com