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ANXA6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 5 bp deletion in exon7.

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Images

Western blot - Human ANXA6 (Annexin-6) knockout HeLa cell lysate (AB257351), expandable thumbnail
  • Western blot - Human ANXA6 (Annexin-6) knockout HeLa cell lysate (AB257351), expandable thumbnail
  • Western blot - Human ANXA6 (Annexin-6) knockout HeLa cell lysate (AB257351), expandable thumbnail
  • Sanger Sequencing - Human ANXA6 (Annexin-6) knockout HeLa cell lysate (AB257351), expandable thumbnail
  • Sanger Sequencing - Human ANXA6 (Annexin-6) knockout HeLa cell lysate (AB257351), expandable thumbnail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 5 bp deletion in exon7.

Alternative names

What's included?

1 Kit
Components
Human ANXA6 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

ANXA6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 5 bp deletion in exon7.

Key facts

Cell type

HeLa

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 5 bp deletion in exon7.

Disease

Adenocarcinoma

Concentration
Loading...

Properties

Gene name

ANXA6

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Annexin-6 also known as ANXA6 is a member of the annexin family of proteins. It has a mass of approximately 68 kDa and is expressed in various tissues with substantial presence in muscle and liver. This protein acts mechanically by binding to membranes in a calcium-dependent manner playing a role in membrane-associated processes such as endocytosis and exocytosis. Annexin-6 can also interact with cytoskeletal elements influencing cell structure and stability.

Biological function summary

Annexin-6 functions as a versatile scaffold that organizes multiprotein complexes. It modulates membrane trafficking processes and participates in signaling pathways within the cell. This protein can influence intracellular calcium homeostasis by interacting with calcium channels and transporters making it an integral part of cellular signaling and communication.

Pathways

Annexin-6 plays a role in the MAPK and PI3K/Akt signaling pathways. It associates with proteins such as Ras and Protein Kinase C impacting cell proliferation survival and differentiation. These interactions highlight the importance of annexin-6 in cellular responses to external stimuli and help regulate various physiological processes in the body.

Associated diseases and disorders

Annexin-6 has been linked to cardiac disorders and cancer. In the context of cardiac health it interacts with proteins like phospholamban and calmodulin to influence heart muscle function and calcium regulation. In cancer annexin-6's involvement in the Akt signaling pathway can affect tumor growth and metastasis often interacting with other proteins like EGFR and HER2. This makes it a significant protein of interest in disease research and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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