Human AP1B1 knockout HEK-293T cell lysate
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AP1B1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 185 bp deletion in exon4 and 1 bp insertion in exon4 and 31 bp deletion in exon4.
View Alternative Names
AP-1 complex subunit beta-1, AP1B1_HUMAN, Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, Beta-1-adaptin, Beta-adaptin 1, Clathrin assembly protein complex 1 beta large chain, Golgi adaptor HA1/AP1 adaptin beta subunit
- Sanger seq
Unknown
Sanger Sequencing - Human AP1B1 knockout HEK-293T cell lysate (AB263089)
Allele-2 : 1 bp insertion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human AP1B1 knockout HEK-293T cell lysate (AB263089)
Allele-1 : 31 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human AP1B1 knockout HEK-293T cell lysate (AB263089)
Allele-3 : 185 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of the AP1B1 extends to ensuring proper cellular trafficking maintaining cell polarity and function within epithelial cells. As part of the AP-1 complex it interacts with other subunits to facilitate the budding of vesicles a critical step in cell function and homeostasis. AP1B1's involvement in intracellular transport underlines its significance in cellular processes that require precise protein localization and distribution.
Pathways
The AP1B1 protein plays an essential role in the clathrin-mediated endocytosis and post-Golgi trafficking pathways. It operates alongside other proteins like clathrin and AP-1 γ subunit coordinating the transport of receptors and other protein complexes to their specific intracellular destinations. This molecular coordination supports processes such as receptor recycling and lysosomal targeting within the cell environment.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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