AP1M1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon1 and 26 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon1 and 26 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Alternative names
AP-1 complex subunit mu-1, AP-mu chain family member mu1A, AP1M1_HUMAN, AP47, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, CLAPM2, CLTNM, Clathrin adaptor protein AP47, Clathrin assembly protein complex 1 medium chain, Clathrin assembly protein complex 1 medium chain 1, Clathrin assembly protein complex AP1 mu subunit, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor AP 1 47 kDa protein, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, HA1 47 kDa subunit, MU 1A, Mu-adaptin 1, Mu1A-adaptin
What's included?
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AP1M1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon1 and 26 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon1 and 26 bp insertion in exon1 and Insertion of the selection cassette in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- AP1M1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AP1M1 also known as Adaptor-related Protein Complex 1 Mu 1 Subunit is a protein with a molecular mass of around 50 kDa. AP1M1 functions as a component of the adaptor protein complex 1 (AP-1). It plays an essential mechanical role in sorting and trafficking proteins by mediating the selection of cargo molecules for transport vesicles. This protein is expressed in multiple tissues with particularly high levels noted in neuronal cells.
- Biological function summary
AP1M1 serves as a critical link in intracellular transport processes. It is part of the AP-1 complex which consists of other subunits like gamma beta and sigma. These complexes work together to ensure proteins move correctly within the cell especially between the trans-Golgi network and endosomes. AP1M1 assists in clathrin-mediated trafficking playing an important role in maintaining cellular functionality by organizing the delivery of membrane proteins and other cellular components.
- Pathways
AP1M1 integrates into the clathrin-coated vesicle pathway and the endocytic pathway. It cooperates with proteins such as clathrin and adaptins including other AP complexes to facilitate the formation of transport vesicles. These pathways are pivotal for protein sorting and the regulation of cellular homeostasis contributing to processes such as receptor recycling and lysosomal enzyme targeting.
- Associated diseases and disorders
Mutations or dysfunction in AP1M1 are linked with neurodegenerative diseases like Alzheimer's disease and some cancers. AP1M1's interaction with proteins like tau in Alzheimer’s illustrates its importance in neurological pathways and synaptic health. In cancer improper protein sorting due to AP1M1 malfunction may influence metastatic behavior as it is connected to proteins involved in cell growth and division.
Product promise
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Terms & Conditions.
3 product images
Sanger Sequencing - Human AP1M1 knockout HeLa cell lysate (ab258783)
Allele-3: Insertion of the selection cassette in exon1
Sanger Sequencing - Human AP1M1 knockout HeLa cell lysate (ab258783)
Allele-1: 25 bp insertion in exon1
Sanger Sequencing - Human AP1M1 knockout HeLa cell lysate (ab258783)
Allele-2: 26 bp insertion in exon1
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