Human AP1M2 knockout HeLa cell lysate
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AP1M2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
AP-1 complex subunit mu-2, AP-mu chain family member mu1B, AP1M2_HUMAN, AP47 2, Adaptor protein complex AP-1 mu-2 subunit, Adaptor-related protein complex 1 mu-2 subunit, Clathrin assembly protein complex 1 medium chain 2, Golgi adaptor HA1/AP1 adaptin mu-2 subunit, HA1 47 kDa subunit 2, HSMU1B, MU 1B, Mu-adaptin 2, Mu1B-adaptin
- Sanger seq
Unknown
Sanger Sequencing - Human AP1M2 knockout HeLa cell lysate (AB263090)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AP1M2 interacts with other subunits to form the AP-1 complex a critical assembly for sorting lysosomal enzymes and trans-Golgi network system function. The AP-1 complex directs the budding of vesicles by interacting with clathrin and cargo membrane proteins. These interactions ensure proper intracellular trafficking contributing to the maintenance of cellular homeostasis. AP1M2’s interactions within the complex indicate its indispensable role in proper membrane protein sorting.
Pathways
AP1M2 contributes significantly to the endocytic pathway and protein transport processes. It is active within the clathrin-mediated endocytosis pathway working closely with proteins such as AP1B1 and AP1G1. These partnerships facilitate the transport of essential molecules from the trans-Golgi network to endosomes and lysosomes influencing cell signaling and nutrient uptake.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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