Human APBB1 (FE65) knockout HEK-293T cell lysate
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APBB1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2.
View Alternative Names
Amyloid beta precursor protein binding family B member 1, Amyloid-beta A4 precursor protein-binding family B member 1, APBB1, RIR, FE65, Protein Fe65
- WB
Lab
Western blot - Human APBB1 (FE65) knockout HEK-293T cell lysate (AB257833)
Lane 1 : Wild-type HEK-293T cell lysate, 20 ug
Lane 2 : APBB1 knockout HEK-293T cell lysate, 20 ug
Lane 3 : SH-SY5Y cell lysate, 20 ug
Lane 4 : A431 cell lysate, 20 ug
False colour image of Western blot : Anti-FE65 antibody [EPR3538] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab91650 was shown to bind specifically to FE65. A band was observed at 65/85 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in APBB1 knockout cell line ab267294 (knockout cell lysate ab257833). To generate this image, wild-type and APBB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human APBB1 (FE65) knockout HEK-293T cell lysate (AB257833)
Homozygous : 4 bp deletion in exon2
Complementary Products
Cell Lines & Lysates
AB267294
Human APBB1 (FE65) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FE65 plays a significant role in neuronal development and cognitive functions. It is part of a protein complex that includes the amyloid precursor protein (APP). This complex is important for the regulation of gene expression and cellular communication. FE65 also affects the nuclear signaling by interacting with the chromatin-remodeling complex. These interactions suggest that FE65 has a hand in influencing transcriptional activities within the cell.
Pathways
FE65 is involved in the APP processing pathway and the development of Alzheimer's disease. It interacts with APP and influences its role in the cleavage and formation of amyloid-beta peptides. These peptides are key factors within the Alzheimer's disease pathway. Moreover FE65 connects with other proteins such as Abl which is involved in cell differentiation and migration processes. Through these pathways FE65 contributes to a broad range of neuronal functions and activities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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