Human APEX2 knockout HEK-293T cell lysate
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(1 Publication)
APEX2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 1.
View Alternative Names
AP endonuclease 2, AP endonuclease XTH2, APE 2, APEX L2, APEX nuclease (apurinic/apyrimidinic endonuclease) 2, APEX nuclease 2, APEX nuclease-like 2, APEX2_HUMAN, Apurinic-apyrimidinic endonuclease 2, Apurinic/apyrimidinic endonuclease 2, Apurinic/apyrimidinic endonuclease like 2, C430040P13Rik, DNA apurinic or apyrimidinic site lyase 2, DNA-(apurinic or apyrimidinic site) lyase 2, EC 4.2.99.18, OTTHUMP00000023390, OTTHUMP00000061908, XTH 2, ZGRF2, Zinc finger GRF type containing 2
- Sanger seq
Unknown
Sanger Sequencing - Human APEX2 knockout HEK-293T cell lysate (AB263091)
Homozygous : 17 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
APEX2 maintains genomic integrity by participating in the base excision repair (BER) pathway where it acts on AP sites facilitating proper DNA repair. It does not function alone but often interacts with other proteins involved in this pathway forming a highly efficient complex. These interactions ensure the correct processing of damaged DNA allowing cells to preserve their genomic content effectively. APEX2's mediation of DNA repair contributes significantly to cell survival and response to DNA-damaging agents.
Pathways
APEX2 finds important roles in the DNA damage response mechanism and oxidative stress response pathways. It collaborates with proteins like XRCC1 and DNA polymerase beta in the BER pathway to safeguard genetic information. These interactions underline its importance in the repair mechanism ensuring accuracy and efficiency in correcting DNA errors. This coordination of pathways highlights APEX2's function in controlling cellular oxidative stress response and maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cell transplantation 33:9636897241296366 PubMed39624898
2024
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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