Human APLNR (APJ Receptor) knockout HeLa cell lysate
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APLNR KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon1 and 1 bp deletion in exon1.
View Alternative Names
AGTRL1, APLNR, G-protein coupled receptor APJ, G-protein coupled receptor HG11, Apelin receptor, APJ, Angiotensin receptor-like 1
- Sanger seq
Unknown
Sanger Sequencing - Human APLNR (APJ Receptor) knockout HeLa cell lysate (AB258306)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human APLNR (APJ Receptor) knockout HeLa cell lysate (AB258306)
Allele-1 : 13 bp deletion in exon1
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The APJ Receptor influences cardiovascular function fluid homeostasis and energy metabolism. It does not form a part of a larger receptor complex but collaborates closely with other proteins within cellular networks. APJ Receptor engagement by apelin or elabela modulates blood pressure cardiac contractility and fluid balance underlying its important role in cardiovascular and renal systems. Therefore this receptor contributes significantly to maintaining physiological homeostasis.
Pathways
The APJ Receptor is integral to the Apelin signaling pathway known to influence cardiac function and embryonic development. It interacts with proteins such as the angiotensin receptor which also participates in regulation of blood pressure. Additionally APJ Receptor involvement in the PI3K-Akt signaling pathway affects cell survival and growth allowing it to intersect with pathways involved in various physiological processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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