Human APOL2 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human APOL2 knockout A549 cell lysate (AB258307)
Homozygous : 4 bp deletion in exon4
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
APOL2 contributes to lipid trafficking and cellular lipid homeostasis. It plays a role in the formation of lipid droplets within cells significant for energy storage and regulation. APOL2 does not function alone but often acts in coordination with other proteins involved in lipid metabolism including members of its own family. This association through protein complexes allows for efficient regulation of lipid movement and storage in cells.
Pathways
Several important pathways intersect with APOL2's functions. It participates in the lipid metabolism pathway which affects energy balance within cells. Another pathway where APOL2 plays a role is the inflammatory response pathway as its expression can influence the response to inflammatory signals. APOL2 shows functional connections with other apolipoprotein family members such as APOL1 through these pathways helping to maintain cellular biochemical equilibrium.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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