Human APPL1 (APPL) knockout HeLa cell lysate
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APPL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
View Alternative Names
AKT2 INTERACTOR, APPL 1, Adapter protein containing PH domain, Adaptor Protein Containing pH Domain, Adaptor protein containing pH domain PTB domain and leucine zipper motif, Adaptor protein containing pH domain PTB domain and leucine zipper motif 1, Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1, DCC-interacting protein 13-alpha, DP13A_HUMAN, Dip13-alpha, KIAA1428, PTB Domain and Leucine Zipper Motif, PTB domain and leucine zipper motif 1, Signaling adaptor protein DIP13alpha
- WB
Lab
Western blot - Human APPL1 (APPL) knockout HeLa cell lysate (AB257836)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : APPL1 knockout HeLa cell lysate (20µg)
Lane 3 : MCF7 cell lysate (20µg)
Lane 4 : HEK-293 cell lysate (20µg)
Lanes 1- 4 : Merged signal (red and green). Green - ab180140 observed at 90 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab180140 Anti-APPL antibody [EPR13569] was shown to specifically react with Anti-APPL in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab265187 (knockout cell lysate ab257836) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Anti-APPL knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-APPL antibody [EPR13569] (<a href='/en-us/products/primary-antibodies/appl-antibody-epr13569-ab180140'>ab180140</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
APPL knockout HeLa cell lysate at 20 µg
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
HEK-293T cell lysate at 20 µg
Predicted band size: 80 kDa
Observed band size: 90 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human APPL1 (APPL) knockout HeLa cell lysate (AB257836)
Homozygous : 2 bp deletion in exon2
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
APPL1 influences cellular processes such as endocytosis and cell proliferation. APPL1 interacts with rab5 forming a component of endosomal signaling complexes. This interaction drives the early endocytic process influencing cell surface receptor trafficking. APPL1 also modulates insulin signaling by binding to proteins like the insulin receptor substrate affecting glucose metabolism and cellular energy balance.
Pathways
APPL1 acts in the AKT signaling pathway and the adiponectin signaling pathway. In the AKT pathway APPL1 operates with proteins like AKT itself and GSK3β contributing to cell survival and growth. It assists the adiponectin pathway by facilitating interactions between adiponectin receptors and downstream effectors impacting lipid metabolism and insulin sensitivity. APPL1's involvement in these pathways highlights its multifaceted regulatory roles in cellular metabolism and response to stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-APPL antibody [EPR13569] (AB180140)](https://content.abcam.com/products/images/appl-antibody-epr13569-ab180140--western-blot-img147153.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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