Human ARAP3 knockout HeLa cell lysate
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ARAP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 2 bp deletion in exon7.
View Alternative Names
ARAP 3, ARF GAP, ARF-GAP RHO-GAP ankyrin repeat and plekstrin homology domains containing protein 3, Ankyrin repeat and plekstrin homology domains containing protein 3, CENTD3, FLJ21065, Phosphoinositide binding protein, PtdIns 3 4 5 P3 binding protein, RHO GAP
- Sanger seq
Unknown
Sanger Sequencing - Human ARAP3 knockout HeLa cell lysate (AB263094)
Allele-2 : 1 bp insertion in exon7
- Sanger seq
Unknown
Sanger Sequencing - Human ARAP3 knockout HeLa cell lysate (AB263094)
Allele-1 : 2 bp deletion in exon7
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARAP3 modulates intracellular signaling pathways that control cytoskeletal rearrangement and cellular adhesion. It acts as part of a protein complex that includes PI3K and is influenced by phosphoinositide binding. This interaction helps facilitate growth factor-induced cell movement. Researchers observe that ARAP3 interacts with cell surface receptors to relay extracellular signals to cytoskeletal components affecting cellular dynamics.
Pathways
ARAP3 participates significantly in the PI3K/Akt pathway mediating membrane trafficking and signal transduction. Its activity impacts the regulation of Rho family GTPases central to controlling cell shape and motility. ARAP3 works together with Arf6 and plays a modulator role linking between actin remodeling and vesicle transport systems. This protein maintains the communication between PI3K signaling and actin reorganization driving cellular responses to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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